benzo a pyrene (MedChemExpress)

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MedChemExpress benzo a pyrene
Benzo A Pyrene, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/benzo+a+pyrene/Benzo%5Ba%5Dpyrene/pm42777927-55-0-15
Average 94 stars, based on 12 article reviews

benzo a pyrene - by Bioz Stars, 2026-10

94/100 stars

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DNA Extraction:

Article Title: FCRL3 as a potential link between Benzo[a]pyrene exposure and primary biliary cholangitis: insights from comparative toxicogenomics and multi-omics analysis.
Article Snippet: Raji cells were cultured in a humidified incubator under a 5% CO2 atmosphere at 37 °C with a specific medium (CM-0189, Procell, China), which consisted of 89% RPMI-1640 [PM150110] + 10% fetal bovine serum [164210] + 1% penicillin-streptomycin [PB180120], and the culture medium was replenished daily. .. DNA extraction and real-time quantitative polymerase chain reaction (RT-qPCR) analysis Raji cells were seeded in specific medium and treated with various concentrations of Benzo[a]pyrene (HY107377, MedChemExpress, China) at 0, 5, 10, 20, 40, and 80μM for 24 h. Following the incubation, the cells were harvested by centrifugation at 1000 × g for 5 min and washed twice with ice-cold phosphate-buffered saline (PBS) for subsequent experiments. .. TRIZOL (R401-01, Vazyme, China) was used to extract total RNA.

Real-time Polymerase Chain Reaction:

Incubation:

Article Title: FCRL3 as a potential link between Benzo[a]pyrene exposure and primary biliary cholangitis: insights from comparative toxicogenomics and multi-omics analysis
Article Snippet: Raji cells were cultured in a humidified incubator under a 5% CO2 atmosphere at 37 °C with a specific medium (CM-0189, Procell, China), which consisted of 89% RPMI-1640 [PM150110] + 10% fetal bovine serum [164210] + 1% penicillin-streptomycin [ PB180120 ], and the culture medium was replenished daily. .. Raji cells were seeded in specific medium and treated with various concentrations of Benzo[a]pyrene (HY-107377, MedChemExpress, China) at 0, 5, 10, 20, 40, and 80μM for 24 h. Following the incubation, the cells were harvested by centrifugation at 1000 × g for 5 min and washed twice with ice-cold phosphate-buffered saline (PBS) for subsequent experiments. .. TRIZOL (R401-01, Vazyme, China) was used to extract total RNA.

Centrifugation:

Article Title: FCRL3 as a potential link between Benzo[a]pyrene exposure and primary biliary cholangitis: insights from comparative toxicogenomics and multi-omics analysis
Article Snippet: Raji cells were cultured in a humidified incubator under a 5% CO2 atmosphere at 37 °C with a specific medium (CM-0189, Procell, China), which consisted of 89% RPMI-1640 [PM150110] + 10% fetal bovine serum [164210] + 1% penicillin-streptomycin [ PB180120 ], and the culture medium was replenished daily. .. Raji cells were seeded in specific medium and treated with various concentrations of Benzo[a]pyrene (HY-107377, MedChemExpress, China) at 0, 5, 10, 20, 40, and 80μM for 24 h. Following the incubation, the cells were harvested by centrifugation at 1000 × g for 5 min and washed twice with ice-cold phosphate-buffered saline (PBS) for subsequent experiments. .. TRIZOL (R401-01, Vazyme, China) was used to extract total RNA.

Saline:

Article Title: FCRL3 as a potential link between Benzo[a]pyrene exposure and primary biliary cholangitis: insights from comparative toxicogenomics and multi-omics analysis
Article Snippet: Raji cells were cultured in a humidified incubator under a 5% CO2 atmosphere at 37 °C with a specific medium (CM-0189, Procell, China), which consisted of 89% RPMI-1640 [PM150110] + 10% fetal bovine serum [164210] + 1% penicillin-streptomycin [ PB180120 ], and the culture medium was replenished daily. .. Raji cells were seeded in specific medium and treated with various concentrations of Benzo[a]pyrene (HY-107377, MedChemExpress, China) at 0, 5, 10, 20, 40, and 80μM for 24 h. Following the incubation, the cells were harvested by centrifugation at 1000 × g for 5 min and washed twice with ice-cold phosphate-buffered saline (PBS) for subsequent experiments. .. TRIZOL (R401-01, Vazyme, China) was used to extract total RNA.

Concentration Assay:

Article Title: LYVE1 and IL1RL1 are mitochondrial permeability transition-driven necrosis-related genes in heart failure.
Article Snippet: Background: Heart failure is linked to increased hospitalization and mortality.. Mitochondrial permeability transition-driven necrosis is associated with cardiovascular diseases, but its role in heart failure is unclear.. This study aimed to identify and validate genes related to mitochondrial permeability transition-driven necrosis in heart failure, potentially leading to new drug targets and signaling pathways.

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